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课题案例:lncRNA通过A信号调节细胞X表型转化

Fig 1 Effect of over-expression A on phenotype modulation in X cells.


(A) Expression of protein A in X cells.

(B) Phenotype modulation of X cells. Increase of protein A could promote cell proliferation and migration by transferring over- expression plasmid into X cells. * 0.01 < p < 0.5; n=3, mean±SD.

Fig 2 Effect of siRNA and inhibitor of A on phenotype modulation in X cells.


(A) Expression of protein A in X cells.

(B) Phenotype modulation of X cells.

(C) Mechanism of signal pathway. Contrary to overexpression of A, knock-down by siRNA and interrupt protein A activating by inhibitor could block phenotype modulation in X cells through relative signals. * 0.01 < p < 0.5; ** p < 0.001; n=3, mean±SD..

Fig 3 Effect of lncRNA mimic and inhibitor on expression of mRNA and protein of A, and phenotype modulation in X cells.


(A) Expression of protein A in X cells.

(B) Phenotype modulation of X cells.

(C) Mechanism of signal pathway. The lncRNA mimic could enhance the ability of cell proliferation and migration by up-regulating A and relative signals in X cells, which lncRNA inhibitor down-regulated. * 0.01 < p < 0.5; ** p < 0.001; n=3, mean±SD.

Fig 4 Modulation of lncRNA to A mRNA measured by dual-luciferase reporter. The lncRNA was proved it targeting to A mRNA. ** p < 0.001; n=3, mean±SD.