课题案例：干细胞移植对病变大鼠的作用
Fig 1 Incubation day 3 and day 7 of isolated stem
cells from SD rats.
Fig 2 Detection of stem cell surface markers.
(A) IF assay.
(B) Flow cytometry detection. It is identified as stem cell by CD m, CD x and Y positive while CD n negative.
Fig 3 Differentiation of stem cell. Osteogenic
differentiation of stem cell detected by Alizarin red, Von kossa and ALP
staining. Adipogenic differentiation of stem cell detected by Oil Red O
staining. It shows the isolated cells could be osteogenic and adipogenic differentiated.
Fig 4 Effect of tail vein transplanted stem cells on
rat pathological tissue investigated by HE and Masson staining. Obviously, stem
cell transplantation had positive effcet on pathological liver tissue of SD
rat.
Fig 5 Inhibition of tail vein transplanted stem cells
on apoptosis of rat pathological tissue detected by TUNEL(transferase-mediated
deoxyuridine triphosphate-biotin nick end labeling) staining.
Fig 6 Effect of tail vein transplanted stem cells on
autophagy of rat pathological tissue observed by transmission electron
microscope. Stem cell may have good benefits on recovery of pathological liver
tissue in SD rat by causing cell autophagy.
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