Fig 1 Effects of chemical A on cancer cell proliferation, migration and invasion.
(A) CCK8 assay.
(B) Wound Healing and Transwell invasion assay. Chemical A inhibited significantly the proliferation, migration and invasion of tumor cells .
Fig 2 Effects of chemical A on cancer cell apoptosis, cycle and MMP (mitochondrial membrane potential). From left to right: Annex V & PI, PI and JC-1 staining. Apoptosis, G0/G1 phase arrest and decrease of MMP was induced by chemical A in tumor cells.
Fig 3 Induction of autophagy by treatment of chemical
A in tumor cells. From left to right: IF detection of LC3, Acridine orange
staining and electron microscope (1500×, 5000×).
Fig 4 Apoptosis and autophagy caused by chemical A
through modulating relative signals in tumor cells.
(A) IF assay
quantitative PCR assay
(C) Western Blot assay. The promotion of apoptosis and
autophagy by chemical A was mediated by protein C.
Fig 5 Inhibition of tumor cell subcutaneous xenograft
growth by chemical A.
(A) Macroscopic appearance of xenografts.
Fig 6 Effects of chemical A treatment on mouse tumor model caused by cancerogenic chemicals. From top to bottom: mouse tissues, HE
staining and IHC assay. Chemical A prevented hepatocellular carcinoma induced
by cancerogenic chemicals remarkably in mice, which may cured pathologically
through protein C signals.
Fig 7 Therapeutical effect of chemical A is mediated
by protein C relative signal pathway. (Assays such as IF, WB and so on)