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课题案例:干细胞移植对病变大鼠的作用

Fig 1 Incubation day 3 and day 7 of isolated stem cells from SD rats.  


Fig 2 Detection of stem cell surface markers.


(A) IF assay.

(B) Flow cytometry detection. It is identified as stem cell by CD m, CD x and Y positive while CD n negative.

Fig 3 Differentiation of stem cell. Osteogenic differentiation of stem cell detected by Alizarin red, Von kossa and ALP staining. Adipogenic differentiation of stem cell detected by Oil Red O staining. It shows the isolated cells could be osteogenic and adipogenic differentiated. 
Fig 4 Effect of tail vein transplanted stem cells on rat pathological tissue investigated by HE and Masson staining. Obviously, stem cell transplantation had positive effcet on pathological liver tissue of SD rat. 


Fig 5 Inhibition of tail vein transplanted stem cells on apoptosis of rat pathological tissue detected by TUNEL(transferase-mediated deoxyuridine triphosphate-biotin nick end labeling) staining. 


Fig 6 Effect of tail vein transplanted stem cells on autophagy of rat pathological tissue observed by transmission electron microscope. Stem cell may have good benefits on recovery of pathological liver tissue in SD rat by causing cell autophagy.